The study of clonal propagation Costus SP. plants for Diabetes prevention purpose.

Result of propagation:

In vitro propagation procedure was established; Samples of Costus stem segments were sterilized with 2% javel and 0.1% HgCl2 for 4 min. Dip the body passages in a solution containing 0.05 mg/l NAA + 1.0 mg/l BAP to disrupt axillary bud dormancy. Addition of 15 mg/l AdSO4 to SH medium + 0.05 mg/l BAP has the ability to stimulate shoot multiplication and shoot growth when culturing stem segments in vitro. Transfer shoots to MS medium + 0.7 mg/l BAP + 1.5 mg/l NAA for in vitro rooting.

In vitro propagation procedure was established; Samples of Costus stem segments were sterilized with 2% javel and 0.1% HgCl2 for 4 min. Dip the body passages in a solution containing 0.05 mg/l NAA + 1.0 mg/l BAP to disrupt axillary bud dormancy. Addition of 15 mg/l AdSO4 to SH medium + 0.05 mg/l BAP has the ability to stimulate shoot multiplication and shoot growth when culturing stem segments in vitro. Transfer shoots to MS medium + 0.7 mg/l BAP + 1.5 mg/l NAA for in vitro rooting.