Paecilomyces sp. is considered one of the most effective nematodicidal fungus. it a genus of phylum Deuteromycota, a insect parasite fungus, has a wide host spectrum and commonly present in nature in both tropical and temperate regions (Thùy, 2004).
P.lilacinus culture medium are Sabouraud Dextrose Agar Yeast + mineral, Sabouraud Dextrose Agar Yeast + Chitin and Potato Dextrose Agar, for a high spore density about 1 – 5 x 108 cfu/ml after 2 days of culture.
Fig 2. P. lilacinus biomass on liquid medium. a) after 24h; b) after 36h; c) after 48h
F3a. P. citri die after spraying Paecilomyces sp. 5 days. F3a. P. citri die after spraying Paecilomyces sp. 7 days.
The fungal hosts include more than 25 families of insects and many other cicadas, as for the nematode host, result of isolation is often Paecilomyceslilacinus, a strain found in soil and vegetation (Jatala, 1986).
Fig4. Nematodes infected with P. lilacinus after 5 days and 7 days
For nematode’s eggs, P. lilacinus have the ability to produce enzymes to break down the shells and eggshells of nematode. Two enzymes important for fungal infection are chitinase and protease. They will contact and destroy the shell of the nematode’s eggs, making it impossible for the eggs to hatch (Khan et al., 2006).
For nematode, P. lilacinus nematode parasites, fungal spores will attach to destroy the shell and infection by the enzymes was produced, then use the nutrients in the host to form mycelium, the fungus will grow and gradually destroy the inside, causing the insect to be completely deformed and then die (Khan et al., 2006).
