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Medicinal Herbs Research 

The biomass process of Taxus wallichiana Zucc callus

Nacentech HCM

Research on the biomass process of Taxus wallichiana Zucc. callus and solutions to improve Taxane content in biomass.

Result:

  • Building the process of biomass Taxus wallichiana Zucc. Callus: Inoculation time to limit browning of samples is 15 days. Culture medium should be pretreated with Acid ascorbic 2% and AgNO3 150 ppm to limit sample browning and stimulate callus formation and increase biomass.
  • Provide a solution to improve the content of Paclitaxel in callus:
  • State 1: Stimulation of callus growth with B5 medium supplemented with 0.1ppm 2,4-D; 0,5ppm 6-BA and 0,5ppm NAA and 150 ppm AgNO3.
  • State 2: Stimulation of Paclitaxel compound accumulation in callus by inoculation of callus on B5 medium supplemented with 0.1ppm 2,4-D; 0,5ppm 6-BA and 0,5ppm NAA and 150 ppm AgNO3 and Methyl jasmonate 5ppm, VOSO4 0,2mM or Methyl jasmonate 5ppm, Salicylic acid 50ppm and VOSO4 0,2mM.
biomass process of Taxus wallichiana
  Fig. Callus cultured on B5 medium supplemented with inducers
  • Building the process of biomass Taxus wallichiana Zucc. Callus: Inoculation time to limit browning of samples is 15 days. Culture medium should be pretreated with Acid ascorbic 2% and AgNO3 150 ppm to limit sample browning and stimulate callus formation and increase biomass.
  • Provide a solution to improve the content of Paclitaxel in callus:
  • State 1: Stimulation of callus growth with B5 medium supplemented with 0.1ppm 2,4-D; 0,5ppm 6-BA and 0,5ppm NAA and 150 ppm AgNO3.
  • State 2: Stimulation of Paclitaxel compound accumulation in callus by inoculation of callus on B5 medium supplemented with 0.1ppm 2,4-D; 0,5ppm 6-BA and 0,5ppm NAA and 150 ppm AgNO3 and Methyl jasmonate 5ppm, VOSO4 0,2mM or Methyl jasmonate 5ppm, Salicylic acid 50ppm and VOSO4 0,2mM.